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Research ArticleArticles

A review of cellular biopreservation considerations during hair transplantation

Aby J. Mathew
Hair Transplant Forum International January 2013, 23 (1) 1-11; DOI: https://doi.org/10.33589/23.1.0001
Aby J. Mathew
Washington, USA
PhD Bothell
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  • For correspondence: amathew@biolifesolutions.com
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    Figure 1.

    Viable recovery of human mesenchymal stem cells following cold storage. Dermal papilla cells share lineage with mesenchymal stem cells. Cell types relevant to hair transplant procedures, such as dermal fibroblasts and epithelial keratinocytes, also exhibit similar post-preservation results to the fluorescent micrographs above (fluorescent micrographs and data reference provided courtesy of BioLife Solutions, Inc.). Cells stored in Normosol (A), Plasma-Lyte (B), and Viaspan (C) exhibit lower cell number and less cell integrity following storage, in comparison to cells preserved in HypoThermosol-FRS (D).

  • Figure 2.
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    Figure 2.

    Differential recovery of metabolic activity in human hair follicles held in HypoThermosol-FRS or normal saline at 2°-8°C. Quantitative readings of a fluorescent metabolic indicator from follicles assayed after Day 6 and Day 9 recovery in cell culture conditions following 1 day of cold storage. Note the lower metabolic viability of follicles stored in saline even a week following the cold storage hold interval.

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    Figure 3.

    Efficacy of hair follicle transplant following 5 days of storage at 4°C in HypoThermosol + liposomal ATP. A single patient study was undertaken in a 71-year-old man, who had both male pattern hair loss and a large area of alopecia on the left temple as a result of radiation for skin cancer. Donor hair was harvested from the occipital scalp, dissected, and stored for 5 days at 4°C. Afterwards, grafts were implanted into alopecic areas (1,200 grafts). The patient was seen at 3, 6, and 9 months for evaluation and final hair counts were performed at 12 months.

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International Society of Hair Restoration Surgery: 23 (1)
Hair Transplant Forum International
Vol. 23, Issue 1
January/February 2013
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A review of cellular biopreservation considerations during hair transplantation
Aby J. Mathew
Hair Transplant Forum International Jan 2013, 23 (1) 1-11; DOI: 10.33589/23.1.0001

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A review of cellular biopreservation considerations during hair transplantation
Aby J. Mathew
Hair Transplant Forum International Jan 2013, 23 (1) 1-11; DOI: 10.33589/23.1.0001
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  • Article
    • Introduction
    • Cellular and Molecular Considerations for Biopreservation
    • Focus on the Preservation Solution for Graft Holding
    • Graft Holding Temperature
    • ATP within Hair Transplantation
    • Further Summary Considerations
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